{"id":1468,"date":"2019-04-25T12:05:45","date_gmt":"2019-04-25T11:05:45","guid":{"rendered":"https:\/\/sites.manchester.ac.uk\/transgenic-unit\/?page_id=1468"},"modified":"2019-05-01T15:33:16","modified_gmt":"2019-05-01T14:33:16","slug":"technology","status":"publish","type":"page","link":"https:\/\/sites.manchester.ac.uk\/genome-editing-unit\/technology\/","title":{"rendered":"Technology"},"content":{"rendered":"

[et_pb_section bb_built=”1″ admin_label=”section” fullwidth=”on” next_background_color=”#000000″ global_module=”322″][et_pb_fullwidth_menu global_parent=”322″ _builder_version=”3.0.106″ menu_id=”7″ submenu_direction=”downwards” fullwidth_menu=”off” background_layout=”dark” module_class=”main-navigation” \/][\/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”off” transparent_background=”off” allow_player_pause=”off” inner_shadow=”off” parallax=”off” parallax_method=”on” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” make_equal=”off” use_custom_gutter=”off” custom_padding=”0px||0px|” custom_css_main_element=”padding-top:0px” module_class=”header-image-section” prev_background_color=”#ffffff” next_background_color=”#ffffff”][et_pb_row allow_player_pause=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” use_custom_gutter=”off” make_equal=”off” custom_margin=”||0px|” custom_padding=”||0px|” module_class=”header-image” background_position=”top_left” background_repeat=”repeat” background_size=”initial”][et_pb_column type=”4_4″][et_pb_image src=”https:\/\/sites.manchester.ac.uk\/genome-editing-unit\/wp-content\/uploads\/sites\/175\/2019\/05\/technology-banner3.jpg” show_in_lightbox=”off” url_new_window=”off” use_overlay=”off” align=”left” always_center_on_mobile=”on” use_border_color=”off” force_fullwidth=”off” custom_margin=”0px|||” animation_direction=”left” show_bottom_space=”off” border_color_all=”#ffffff” _builder_version=”3.17.6″ \/][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”off” transparent_background=”off” allow_player_pause=”off” inner_shadow=”off” parallax=”off” parallax_method=”on” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” make_equal=”off” use_custom_gutter=”off” custom_padding=”||0px|” prev_background_color=”#ffffff” next_background_color=”#ffffff”][et_pb_row allow_player_pause=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” use_custom_gutter=”off” make_equal=”off” custom_margin=”||0px|” custom_padding=”||0px|” module_class=”breadcrumbs-row” background_position=”top_left” background_repeat=”repeat” background_size=”initial”][et_pb_column type=”4_4″][et_pb_text admin_label=”Breadcrumbs (do not remove)” background_layout=”light” text_orientation=”left” use_border_color=”off” border_color=”#ffffff” border_style=”solid” module_class=”breadcrumbs” custom_css_main_element=”border-bottom: 1px solid #ececed;” module_alignment=”left” background_position=”top_left” background_repeat=”repeat” background_size=”initial”]<\/p>\nGenome Editing Unit<\/a><\/span><\/span>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”on” transparent_background=”off” allow_player_pause=”off” inner_shadow=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” parallax_2=”off” parallax_method_2=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” make_equal=”off” use_custom_gutter=”on” gutter_width=”2″ prev_background_color=”#ffffff” next_background_color=”#1d1d1d”][et_pb_column type=”1_3″][et_pb_sidebar admin_label=”Left Nav Sidebar” area=”et_pb_widget_area_1″ orientation=”left” remove_border=”on” background_layout=”light” module_id=”left-nav-menu” show_border=”off” \/][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″][et_pb_row_inner admin_label=”Row”][et_pb_column_inner type=”4_4″ saved_specialty_column_type=”2_3″][et_pb_text admin_label=”Text: Technology intro and CRISPR-Cas9″ background_layout=”light” text_orientation=”left” use_border_color=”off” module_alignment=”left” background_position=”top_left” background_repeat=”repeat” background_size=”initial” _builder_version=”3.17.6″]<\/p>\n

Technology<\/h1>\n

In the Genome Editing Unit (GEU) we are committed to integrating and applying the latest cutting edge molecular technologies. In particular we have become the hub of genome editing activity at The University of Manchester.<\/p>\n

We have the capacity to apply our technologies according to your needs, to generate novel experimental systems, or perhaps even help to develop our own technologies<\/a> to address your biological questions.<\/p>\n

Between us, we have decades of combined experience in molecular biology. As well as CRISPR, we are able to develop a wide variety of molecular constructs, including plasmids, viral vectors and recombinant bacterial artificial chromosomes according to your needs.<\/p>\n

CRISPR-Cas9<\/h2>\n

We have incorporated the revolutionary technology CRISPR-Cas9 into our services portfolio.<\/p>\n

This is a re-purposed bacterial immune system, which allows us to target and break specific DNA sequences in living cells targets. We can then exploit DNA repair to make desired changes to the genome of your experimental system.<\/p>\n

Genomic changes<\/h3>\n

These changes can range from simple gene knockouts (KO), by random disruption of a few DNA sequences, to more precise \u2018knock in\u2019 genetic changes, such as specific DNA base changes or gene tagging with small tags or fusion genes,\u00a0including fluorescent proteins, degron domains, or proximity labelling enzymes, etc.<\/p>\n

[\/et_pb_text][et_pb_video _builder_version=”3.17.6″ src=”https:\/\/youtu.be\/4YKFw2KZA5o” \/][et_pb_text admin_label=”Text: Other applications” _builder_version=”3.17.6″]<\/p>\n

Other applications<\/h3>\n

We also help with derivative applications of CRISPR-Cas9.<\/p>\n

In particular CRISPRa and CRISPRi, in which the\u00a0Cas9 has been blunted and fused to transcriptional activators or repressors, that can be used to selectively turn endogenous target genes on or off.<\/p>\n

[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section bb_built=”1″ admin_label=”Connect Title” custom_padding=”0px||0px|” _builder_version=”3.0.90″ background_color=”#1d1d1d” module_class=”footer-section” prev_background_color=”#ffffff” global_module=”380″][et_pb_row admin_label=”row” global_parent=”380″ locked=”off” allow_player_pause=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” parallax_2=”off” parallax_method_2=”off” parallax_3=”off” parallax_method_3=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” use_custom_gutter=”off” make_equal=”off” module_id=”footer-row” _builder_version=”3.0.89″][et_pb_column type=”1_3″][et_pb_text admin_label=”Contact Us Title” global_parent=”380″ background_layout=”dark” text_text_color=”#ffffff” parent_locked=”off” background_position=”top_left” background_repeat=”repeat” background_size=”initial”]<\/p>\n

Contact Us<\/h4>\n

[\/et_pb_text][et_pb_text admin_label=”Contact Us” global_parent=”380″ text_text_color=”#aaaaaa” parent_locked=”off” background_layout=”light” text_orientation=”left” use_border_color=”off” module_class=”contact-us” background_position=”top_left” background_repeat=”repeat” background_size=”initial” _builder_version=”3.17.6″]<\/p>\n

genome.editing@manchester.ac.uk<\/a><\/p>\n

\u00a0<\/p>\n

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Find Us<\/h4>\n

[\/et_pb_text][et_pb_text admin_label=”Address” global_parent=”380″ background_layout=”dark” text_orientation=”left” use_border_color=”off” module_id=”address” parent_locked=”off” background_position=”top_left” background_repeat=”repeat” background_size=”initial” _builder_version=”3.17.6″]<\/p>\n

A V Hill Building
The University of Manchester
Upper Brook Street
Manchester
M13 9PT
UK<\/a><\/p>\n

\u00a0<\/p>\n

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Connect With Us<\/h4>\n

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