{"id":1479,"date":"2019-04-25T12:37:07","date_gmt":"2019-04-25T11:37:07","guid":{"rendered":"https:\/\/sites.manchester.ac.uk\/transgenic-unit\/?page_id=1479"},"modified":"2019-05-07T09:43:50","modified_gmt":"2019-05-07T08:43:50","slug":"our-services","status":"publish","type":"page","link":"https:\/\/sites.manchester.ac.uk\/genome-editing-unit\/our-services\/","title":{"rendered":"Our services"},"content":{"rendered":"

[et_pb_section bb_built=”1″ admin_label=”section” fullwidth=”on” next_background_color=”#000000″ global_module=”322″][et_pb_fullwidth_menu global_parent=”322″ _builder_version=”3.0.106″ menu_id=”7″ submenu_direction=”downwards” fullwidth_menu=”off” background_layout=”dark” module_class=”main-navigation” \/][\/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”off” transparent_background=”off” allow_player_pause=”off” inner_shadow=”off” parallax=”off” parallax_method=”on” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” make_equal=”off” use_custom_gutter=”off” custom_padding=”0px||0px|” custom_css_main_element=”padding-top:0px” module_class=”header-image-section” prev_background_color=”#ffffff” next_background_color=”#ffffff”][et_pb_row allow_player_pause=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” use_custom_gutter=”off” make_equal=”off” custom_margin=”||0px|” custom_padding=”||0px|” module_class=”header-image” background_position=”top_left” background_repeat=”repeat” background_size=”initial”][et_pb_column type=”4_4″][et_pb_image src=”https:\/\/sites.manchester.ac.uk\/genome-editing-unit\/wp-content\/uploads\/sites\/175\/2019\/04\/technology-banner.jpg” show_in_lightbox=”off” url_new_window=”off” use_overlay=”off” align=”left” always_center_on_mobile=”on” use_border_color=”off” force_fullwidth=”off” custom_margin=”0px|||” animation_direction=”left” show_bottom_space=”off” border_color_all=”#ffffff” _builder_version=”3.17.6″ \/][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”off” transparent_background=”off” allow_player_pause=”off” inner_shadow=”off” parallax=”off” parallax_method=”on” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” make_equal=”off” use_custom_gutter=”off” custom_padding=”||0px|” prev_background_color=”#ffffff” next_background_color=”#ffffff”][et_pb_row allow_player_pause=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” use_custom_gutter=”off” make_equal=”off” custom_margin=”||0px|” custom_padding=”||0px|” module_class=”breadcrumbs-row” background_position=”top_left” background_repeat=”repeat” background_size=”initial”][et_pb_column type=”4_4″][et_pb_text admin_label=”Breadcrumbs (do not remove)” background_layout=”light” text_orientation=”left” use_border_color=”off” border_color=”#ffffff” border_style=”solid” module_class=”breadcrumbs” custom_css_main_element=”border-bottom: 1px solid #ececed;” module_alignment=”left” background_position=”top_left” background_repeat=”repeat” background_size=”initial”]<\/p>\nGenome Editing Unit<\/a><\/span><\/span>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”on” transparent_background=”off” allow_player_pause=”off” inner_shadow=”off” parallax=”off” parallax_method=”on” parallax_1=”off” parallax_method_1=”off” parallax_2=”off” parallax_method_2=”off” make_fullwidth=”off” use_custom_width=”off” width_unit=”on” make_equal=”off” use_custom_gutter=”on” gutter_width=”2″ prev_background_color=”#ffffff” next_background_color=”#1d1d1d”][et_pb_column type=”1_3″][et_pb_sidebar admin_label=”Left Nav Sidebar” area=”et_pb_widget_area_1″ orientation=”left” remove_border=”on” background_layout=”light” module_id=”left-nav-menu” show_border=”off” \/][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″][et_pb_row_inner admin_label=”Row”][et_pb_column_inner type=”4_4″ saved_specialty_column_type=”2_3″][et_pb_text admin_label=”Text: Our services intro” background_layout=”light” text_orientation=”left” use_border_color=”off” module_alignment=”left” background_position=”top_left” background_repeat=”repeat” background_size=”initial” _builder_version=”3.17.6″]<\/p>\n

Our services<\/h1>\n

We offer genomic editing services ranging from transgenic modifications to cell engineering.<\/p>\n

[\/et_pb_text][et_pb_accordion admin_label=”Accordion: Services” use_border_color=”off” border_color_all=”#ffffff” _builder_version=”3.17.6″][et_pb_accordion_item title=”Transgenic mice” _builder_version=”3.17.6″ use_background_color_gradient=”off” background_color_gradient_start=”#2b87da” background_color_gradient_end=”#29c4a9″ background_color_gradient_type=”linear” background_color_gradient_direction=”180deg” background_color_gradient_direction_radial=”center” background_color_gradient_start_position=”0%” background_color_gradient_end_position=”100%” background_color_gradient_overlays_image=”off” parallax=”off” parallax_method=”on” background_size=”cover” background_position=”center” background_repeat=”no-repeat” background_blend=”normal” allow_player_pause=”off”]<\/p>\n

Our original and flagship service is the generation of custom transgenic mouse models, tailored to researcher needs.<\/p>\n

We offer a complete service from concept to germline confirmed transgenic line, including construct design, preparation and purification of targeting reagents, generation of the transgenic mouse through embryo microinjection, and confirmation of germline transmission.<\/p>\n

We also offer a number of supporting services including mouse colony cryopreservation and the import\/rederivation of colonies through in vitro<\/em> fertilization (IVF) and embryo transfer.<\/p>\n

Importantly, we use our expertise to advise and intellectually contribute to project requirements and maintain a continuous dialogue to understand often-changing needs.<\/p>\n

If you are interested in generating a custom transgenic mouse model, or any of the other services described, please contact us at genome.editing@manchester.ac.uk<\/a>.<\/p>\n

Internal users can visit our PPMS site <\/a>for pricing information<\/p>\n

CRISPR-Cas9<\/strong><\/p>\n

Strategies mainly focus on CRISPR-Cas9 genome editing, which allows targeted mutagenesis in the single cell embryo. We apply CRISPR-Cas9 to generate a range of endogenous genetic changes, including gene KO, genomic deletions, point mutations, conditional alleles and reporter gene tagging.<\/p>\n

\"CRISPR-Cas9<\/p>\n

Other transgenesis techniques<\/strong><\/p>\n

We have experience with a range of alternative transgenesis techniques, including recombinant DNA approaches such as plasmids and Bacterial artificial chromosomes (BACs), <\/strong>which can be genome integrated in random fashion by linear DNA pro-nuclear injection or piggybac transposase mediated single copy integration<\/p>\n

We have also built viral vectors for use in in vivo, including AAV-Cre and AAV-CRISPR that allow you to perform genetic manipulations without creating a new transgenic line and on different backgrounds.<\/p>\n

 <\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”Cell engineering” _builder_version=”3.17.6″ use_background_color_gradient=”off” background_color_gradient_start=”#2b87da” background_color_gradient_end=”#29c4a9″ background_color_gradient_type=”linear” background_color_gradient_direction=”180deg” background_color_gradient_direction_radial=”center” background_color_gradient_start_position=”0%” background_color_gradient_end_position=”100%” background_color_gradient_overlays_image=”off” parallax=”off” parallax_method=”on” background_size=”cover” background_position=”center” background_repeat=”no-repeat” background_blend=”normal” allow_player_pause=”off”]<\/p>\n

CRISPR-Cas9 can be used in a variety of different ways to  build representative culture models for research, or to  perturb gene networks.<\/p>\n

Genetically engineered cells<\/strong><\/p>\n

\"CancerWe can help make a range of CRISPR modified cell types, including:<\/p>\n