Sustainability
Improving sustainability in research laboratories
Key steps
Plastic pollution and carbon (-equivalent) emissions are key risk factors for our environment that are caused or massively enhanced by human activity. Without doubt, research work in laboratories and the waste produced contribute to both risk factors, often in avoidably excessive ways. If you want to help reverse this trend, a number of aspects need to be considered including the right motivation, awareness of strategies, their implementation, and dissemination.
More details on our key steps
- Accepting responsibility and ownership – Do you and people around you care and feel motivated to invest time and energy?
- Awareness of the possible strategies; these are in crude terms:
- Reduce / Reuse – reduce litter production and energy-intense procedures by revising your routine procedures; reduce plastic waste which can often be done through reuse
- Replace – where possible replace plastics by biodegradable plastics or less harmful materials; replace harmful chemicals where possible
- Recycle / Remove sensibly – where litter is unavoidable, champion recycling procedures or cleaner ways of waste disposal (e.g. biogas plants rather than landfill)
- Taking initiative & implementation – getting started requires time and energy and often leadership; it involves
- gaining the necessary knowledge to be able to make informed decisions
- being creative & innovative in revising procedures
- convincing others to enage on this path
- Sharing good practice – you may want to go beyond your own contribution and generate more impact by inspiring others to follow suit; this may involve making all required information easily accessible, engaging others at your institution in similar practices, involve sustainability in your teaching, being prepared to engage in outreach
Potential barriers
However, you need to be realistic from start; a number of aspects may stand in the way which you need to be aware of, and which might require flexible solutions and often extra initiative. These concern motivation, organisational support, cost & time investment, lack of technology or infrastructure, rules & regulations and the maintenance of standards.
More on potential barriers
- Motivation – achieving team spirit valued by line managers is the ideal scenario on path to sustainability; however, don’t be surprised if you encounter lack of motivation within your research group or line management
- Organisational support & incentives – you may encounter little institutional support; this said, more and more organisations introduce frameworks promoting sustainability, acknowledge individual initiatives, or support facilitating pipelines and resources; external organisations, such as funders, emphasise sustainability to incentivise researchers and organisations (whereas national and international legal frameworks remain unfortunately poor and undecisive);
- Cost & time investment – often sustainable processes can be money-saving at consumable and energy levels, but they might involve more work (e.g. cleaning in reuse scenarios) – and salaries are usually costly; if sustainability involves extra net cost, this might become a barrier
- Lack of technology or infrastructure – to give some examples: (1) of the main plastics used in laboratories, the recycling of PET or polypropylene seems far easier than polystyrene; there might not be any companies in your area that recycle Ps; (2) you may want to use biodegradable plastics, but suitable variants might simply not be on the market or are far too expensive
- Rules & regulations – rules and regulations often have to balance competing interests; for example, biosecurity or health & safety rules and the way they are interpreted by safety officers can stand in the way of more sustainable processes; this said, sometimes these conflicts can be solved with persuasion & well-substantiated argumentation
- Maintaining standards – achieving high standards of sustainability in laboratories can be a satisfying and motivating process; maintaining these standards is a very different challenge and requires stamina, discipline, constant reminders of lab members and induction of newcomers; you may face fatigue if you are investing extra hours to carry out necessary procedures (e.g. cleaning for reuse)
In a nutshell, the task of approaching sustainable research is challenging and requires time, effort, dedication and stamina, persuation and lobbying. It will be easier the more of us contribute and the more governments and institutions stand behind these efforts to generate favourable conditions and infrastructure. Here we aim to facilitate this process by sharing good practice, ideas and instructions – not only for Drosophila laboratories but also beyond.
Improving sustainability in Drosophila research laboratories
Like all labortories working with model organisms, also Drosophila laboratories have to consider two major activities: the actual experimental work and the husbandry of fly stocks.
Improving sustainability of the experimental work
This task will depend on the nature of the research. For example, studies by Weber and colleagues found that a cell & tissue culture laboratories produces ~7 times more plastic waste than a standard molecular biology laboratory, with likely little prospect of reuse – this poses very different research-specific challenges. Our literature collections below lists many examples of articles providing ideas and inititiatives to address these challenges.
Improving sustainability of Drosophila husbandry
This task involves glass or plastic containers used for keeping flies. These containers are can be subject to single-use or re-use. As suggested by our own analysis, there are choices to be taken in either scenario that will have impact on sustainability. The pros and cons of these various procedures and their econimc and energy costs are discussed in our paper.
More on improving husbandry
- material: glass, polystyrene, polyethylene, (biodegradable?)
- inactivating flies: freezing, autoclaving
- re-use procedures: hand- or machine wash, choice of detergent, form of decontamination
- choice of waste removal: landfill, incineration (high or low temperature), plastic recycling, organic waste disposal into the sewer

Procedures used in different fly laboratories
In the following we will collate a commented list of procedures that different fly laboratories have adopted and want to share as good practice, hopefully giving you some ideas what procedures to implement and how to implement them:
- Saroj Saurya (Oxford) : Protocol for washing and reusing vials, bottles, and flugs
Email: saroj.saurya@path.ox.ac.uk
A list of sustainability-focussed publications & online resources
As indicated in (A) above, awareness of possible strategies with their underlying rationales and calculations is not straightforward and has to develop over time by gaining the necessary knowledge and understanding. To facilitate the process, we have listed publications and online resources that we are aware of and found helpful (please, let us know any suggestions you may have!).
Sustainability initiatives and case reports concerning research laboratories
- Alves, J., Sargison, F. A., Stawarz, H., Fox, W. B., Huete, S. G., Hassan, A., McTeir, B., Pickering, A. C. (2021a) A case report: insights into reducing plastic waste in a microbiology laboratory. Access Microbiology 3
- Benito-Garagorri, E., Hasenfeld, P. (2023) Do we really need that many tip boxes? How a simple question can lead to greener omics. Blog post in “embl.org/Sustainability”
- Campion, C., Robertson, L., Stansfield, I., Speirs, V. (2025) Towards greener and more sustainable pre-clinical oncology research. BJC Reports 3, 4
- Challiner, M., Saurya, S., Patel, S., Raff, J. W., Fostier, M., Prokop, A. (2025) Towards more sustainable research: reducing the environmental impact when working with Drosophila. Genetics iyaf114
- Estevez-Torres, A., Gauffre, F., Gouget, G., Grazon, C., Loubet, P. (2024) Carbon footprint and mitigation strategies of three chemistry laboratories. Green Chem 26, 2613-2622
- Farley, M. (2022) How green is your science? The race to make laboratories sustainable. Nat Rev Mol Cell Biol 23, 517
- Farley, M., Nicolet, B. P. (2023) Re-use of laboratory utensils reduces CO2 equivalent footprint and running costs. PLoS ONE 18, e0283697 — https://doi.org/10.1371/journal.pone.0283697
- Fostier, M., Grady, R. (2020) University of Manchester School of Biological Sciences launches a blueprint for drastic laboratory plastic reduction. Biochem (Lond) 42, 43
- Freese, T., Elzinga, N., Heinemann, M., Lerch, M. M., Feringa, B. L. (2024) The relevance of sustainable laboratory practices. RSC Sustainability 2, 1300-36
- Groenewegen, A., Wijnhoven, A., Janmaat, J., de Kort, M., Dekkers, F. (2024) Guidelines for socially responsible (bio) medical research. UMC Utrecht
- Kampmann, M. L., Tfelt-Hansen, J., Borsting, C. (2024) Cleaning protocols in forensic genetic laboratories. Int J Legal Med
- Kilcoyne, J., Bogan, Y., Duffy, C., Hollowell, T. (2022) Reducing environmental impacts of marine biotoxin monitoring: A laboratory report. PLOS Sustain Transform 1, e0000001
- König, L., Pribasnig, T. (2021) Plastic recycling in Austria: we wanted to recycle our plastic waste in the lab but had no idea about its fate. Blog post in “green labs austria,”.
- Kuntin, D. (2018) How to… reduce your lab’s plastic waste. Biologist 65, 28-31
- Merck (2024) Our approach to sustainable product design. Blog post in “merckgroup.com”
- Meyn, S. M., Ramirez-Aguilar, K. A., Gregory, C. W., Mische, S., Ott, A. W., Sol-Church, K., Sturges, M., Taatjes, D. J. (2023) Addressing the environmental impact of science through a more rigorous, reproducible, and sustainable conduct of research. J Biomol Tech 33
- Ow, C., Taylor-Hearn, I., Wunderley, L., Taylor, S., Pool, M., Fostier, M. (2025) Sustainable SDS-PAGE and Western blotting: cutting plastic, not corners. The Biochemist 47, 3-8
- Ragazzi, I., Farley, M., Jeffery, K., Butnar, I. (2023) Using life cycle assessments to guide reduction in the carbon footprint of single-use lab consumables. PLoS Sustain Transform 2, e0000080
- Rizan, C., Bhutta, M. F., Reed, M., Lillywhite, R. (2021) The carbon footprint of waste streams in a UK hospital. J Clean Prod 286, 125446
- Smith, W. V., Bebbington, A., Sircar, R., Pulver, S. R. (2025) Doctoral students as carbon accountants: calculating carbon costs of a PhD in neuroscience. bioRxiv, 2025.01.20.633775
- Taylor-Hearn, I. (2023) Top tips from Gold LEAF champions. Blog post in “BMH Social Responsibility”
- Trusler, E. C., Davies, M., Spurrier, B., Gould, S. J. (2024) Reusable glassware for routine cell culture—a sterile, sustainable and affordable alternative to single-use plastics. Front Sustain 5, 1447236
- Urbina, M. A., Watts, A. J. R., Reardon, E. E. (2015) Labs should cut plastic waste, too. Nature 528, 479
- Wattam, K. J., Todd, M. A. M., Groth, A. (2024) Implementing sustainable science in the lab. Nat Cell Biol 26, 2-3
- Weber, P. M., Michelsen, C., Kerou, M. (2025) What’s in our bin? EMBO Rep 26, 297-302
- Winter, N., Marchand, R., Lehmann, C., Nehlin, L., Trapannone, R., Rokvić, D., Dobbelaere, J. (2023) The paradox of the life sciences: How to address climate change in the lab. EMBO Rep 24, e56683
- Zikhathile, T., Atagana, H., Bwapwa, J., Sawtell, D. (2022) A review of the impact that healthcare risk waste treatment technologies have on the environment. Int J Environ Res Public Health 19, 11967
Relevant technologies
- CARBIOS (2024) CARBIOS celebrates the groundbreaking of its PET biorecycling plant, a world first, with its partners. Blog post
- Chen, G., Li, J., Sun, Y., Wang, Z., Leeke, G. A., Moretti, C., Cheng, Z., Wang, Y., Li, N., Mu, L., Li, J., Tao, J., Yan, B., Hou, L. a. (2024) Replacing traditional plastics with biodegradable plastics: impact on carbon emissions. Engineering 32, 152-162
- Sever, L., Decorte, M. (2024) From waste to water: Biogases’ contribution to a climate-resilient water system. Blog post in “European Biogas Association”
- Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y., Oda, K. (2016) A bacterium that degrades and assimilates poly(ethylene terephthalate). Science 351, 1196-1199
Relevant background information and procedures
- Chamas, A., Moon, H., Zheng, J., Qiu, Y., Tabassum, T., Jang, J. H., Abu-Omar, M., Scott, S. L., Suh, S. (2020) Degradation rates of plastics in the environment. ACS Sustain Chem Eng 8, 3494-511
- Department for Environment Food & Rural Affairs (2022) Experimental statistics on the carbon impact of waste from households managed by local authorities in England.
- Dowen, J., Dowen, S., Hammond, I., Hill, T., Pitcairn, N., Webb, J. (2018) Evaluation of the climate change impacts of waste incineration in the United Kingdom. pp. 56
- ecoinvent (2003) Life Cycle Assessment (LCA). Last accessed: 01 June
- eurostat (2023) Glossary: carbon dioxide equivalent. Last accessed: 16/08/2023
- He, D., Hu, H., Jiao, F., Zuo, W., Liu, C., Xie, H., Dong, L., Wang, X. (2023) Thermal separation of heavy metals from municipal solid waste incineration fly ash: A review. Chem Eng J 467, 143344
- Hindle, C. Polypropylene (PP). Blog post
- Hogue, C. (2022) Chemical recycling of plastic gets a boost in 18 US states—but environmentalists question whether it really is recycling. Chem Eng News 100
- Isobe, A., Azuma, T., Cordova, M. R., Cózar, A., Galgani, F., Hagita, R., Kanhai, L. D., Imai, K., Iwasaki, S., Kako, S. i., Kozlovskii, N., Lusher, A. L., Mason, S. A., Michida, Y., Mituhasi, T., Morii, Y., Mukai, T., Popova, A., Shimizu, K., Tokai, T., Uchida, K., Yagi, M., Zhang, W. (2021) A multilevel dataset of microplastic abundance in the world’s upper ocean and the Laurentian Great Lakes. Microplast Nanoplast 1, 16
- Lau, W. W. Y., Shiran, Y., Bailey, R. M., Cook, E., Stuchtey, M. R., Koskella, J., Velis, C. A., Godfrey, L., Boucher, J., Murphy, M. B., Thompson, R. C., Jankowska, E., Castillo Castillo, A., Pilditch, T. D., Dixon, B., Koerselman, L., Kosior, E., Favoino, E., Gutberlet, J., Baulch, S., Atreya, M. E., Fischer, D., He, K. K., Petit, M. M., Sumaila, U. R., Neil, E., Bernhofen, M. V., Lawrence, K., Palardy, J. E. (2020) Evaluating scenarios toward zero plastic pollution. Science 369, 1455-61
- Leslie, H. A., van Velzen, M. J. M., Brandsma, S. H., Vethaak, A. D., Garcia-Vallejo, J. J., Lamoree, M. H. (2022) Discovery and quantification of plastic particle pollution in human blood. Environ Int 163, 107199
- Mortula, M. M., Atabay, S., Fattah, K. P., Madbuly, A. (2021) Leachability of microplastic from different plastic materials. J Environ Manage 294, 112995
- Open Access News (2022a) “Advanced recycling” not the answer to plastic pollution crisis. Last accessed: 02 Sep 2023
- Pan, X., Yan, J., Xie, Z. (2013) Detoxifying PCDD/Fs and heavy metals in fly ash from medical waste incinerators with a DC double arc plasma torch. J Environ Sci 25, 1362-7
- Pandey, P., Dhiman, M., Kansal, A., Subudhi, S. P. (2023) Plastic waste management for sustainable environment: techniques and approaches. Waste Dispos Sustain Energy 5, 205-22
- Rogers, T. (2015) Everything You Need To Know About Polystyrene (PS). Blog post in “Creative Mechanisms Blog”
- Shen, M., Hu, T., Huang, W., Song, B., Qin, M., Yi, H., Zeng, G., Zhang, Y. (2021) Can incineration completely eliminate plastic wastes? An investigation of microplastics and heavy metals in the bottom ash and fly ash from an incineration plant. Sci Total Environ 779, 146528
- ThermoFisher, Plastic Materials Selection. Last accessed: 04 Sep 2023
- Uy, B., Read, H., van de Pas, S., Marnane, R., Casu, F., Swift, S., Wiles, S. (2022) The efficacy of commercial decontamination agents differs between standardised test settings and research laboratory usage for a variety of bacterial species. PeerJ, 10:e13646
- WestLab (2017) Difference between polystyrene (PS) & polypropylene (PP) with resistance to acids/bases/chemicals? Blog post
- Wojnowska-Baryła, I., Bernat, K., Zaborowska, M. (2022) Plastic waste degradation in landfill conditions: the problem with microplastics, and their direct and indirect environmental effects. Int J Environ Res Public Health 19, 13223
- Yang, Z., Lü, F., Zhang, H., Wang, W., Shao, L., Ye, J., He, P. (2021) Is incineration the terminator of plastics and microplastics? J Hazard Mater 401, 123429
- Zhao, L., Zhang, F.-S., Chen, M., Liu, Z., Wu, D. B. J. (2010) Typical pollutants in bottom ashes from a typical medical waste incinerator. J Hazard Mater 173, 181-5
Guidelines, political developments
- American Committee of Medical, E., American Society of Tropical, M., Hygiene (2019) Arthropod Containment Guidelines, Version 3.2. Vector-Borne and Zoonotic Diseases 19, 152-173
- Department Environment Food & Rural Affairs (2022) UK leads the way on ending plastic pollution
- Departments for Energy Security and Net Zero and for Business Energy & Industrial Strategy (2023) Research and analysis – Greenhouse gas reporting: conversion factors 2023. Last accessed: 16/08/2023
- Health & Safety Executive Guidance from the Scientific Advisory Committee on Genetic Modification.
- Intergovernmental Paen on Climate Change (2014) Climate change 2014: synthesis report – contribution of working groups I, II and III to the 5th assessment report of the IPCC. IPCC
- MIT Office of Sustainability: Decarbonize our campus. Last accessed: 16 Sept
- National Health Service (2023) NHS clinical waste strategy. pp. 27
- NIH Guidelines (2024) NIH guidelines for research involving recombinant or synthetic nucleic acid molecules National Institute of Health
- Nordahl, S. L., Devkota, J. P., Amirebrahimi, J., Smith, S. J., Breunig, H. M., Preble, C. V., Satchwell, A. J., Jin, L., Brown, N. J., Kirchstetter, T. W., Scown, C. D. (2020) Life-cycle greenhouse gas emissions and human health trade-offs of organic waste management strategies. Environ Sci Technol 54, 9200-9
- OECD (2022) Plastic pollution is growing relentlessly as waste management and recycling fall short, says OECD. Last accessed: 30/08/2023
- Open Access News (2022b) The UK to end plastic pollution by 2040. Last accessed: 02 Sep 2023
- UK Government (2021) Environment Act 2021.
- Weber, P., Bendiscioli, S., Wallon, G., von Ahsen, U., de Beaufort, A., Boland, M., Dekkers, F., Dunon-Bluteau, D., Farley, M., Fox, A., Guillot, S., Lannelongue, L., Łazarowicz-Kowalik, M., Rouse, B., Samuel, G., Sanchis, T., Simon, S., Tata, M., vanderGoot, G., Watt, F. (2024) The Heidelberg Agreement on Environmental Sustainability in Research Funding (1.0). Zenodo
- Wellcome Trust (2024) Concordat for the Environmental Sustainability of Research and Innovation Practice