IntroductionThis post is to help you understand the technologies involved in LC-MS systems. LC-MS systems contain or are configured with mixtures of different types of technologies that give each a different capability. Understanding the different technologies helps...
Proteins are complex and dynamic three dimensional molecules that work in groups and can change each other. Proteins interact with themselves and other proteins via different forms of intra (within the same protein) and inter (between proteins) molecular forces that...
When trying to remove one class of molecules from another then you need to look for chemical characteristics that can be used to differentiate between them. In the case of the removal of lipids and detergents from protein samples then the best opportunity for success...
We have had increasing evidence that non-peptide molecules are coming through our sample prep techniques and affecting the LC-MS systems. We are pretty sure about the effect of triton and other detergents such as CHAPS but similar effects from samples that do not...
To solubilise proteins you need to break proteins apart (see guide to protein-protein interactions for details on the forces that keep proteins together) and keep them apart. Breaking proteins apartBreaking proteins apart has two main components, physical disruption...
There are a number of ways in which mass spectrometry can be used to identify and characterise proteins but the method used in Bio-MS is the most common method using fragmentation data from peptides. As well as scanning the masses and intensities of the ions present a...
Recent Comments